In Vitro Embryo Production: Overview

Abstract

Reproductive biotechnologies, also known as assisted reproductive technologies (ART), have undergone significant development over time, reaching a remarkable level of evolution in the 20th century. Currently, several biotechnologies stand out, among which Artificial Insemination (AI), widely spread globally, Embryo Production (EP) both in vivo and in vitro for embryo transfer, Intracytoplasmic Sperm Injection (ICSI), sexing, among other innovations. In Vitro Production (IVP) of embryos has emerged as the biotechnology that has experienced the greatest development and evolution in recent decades, covering various fields such as research in assisted human reproduction, obtaining transgenic animals for biomedicine, preservation of endangered species and conservation of animals of high genetic value. This research aims to analyze the relevance of IVP, as well as the fundamental elements that make up this complex process. IVP of embryos involves several stages, from oocyte procurement, oocyte selection and in vitro maturation, sperm preparation, in vitro fertilization, to the culture of the resulting zygotes to blastocysts. These blastocysts, once transferable to recipients, culminate in the birth of a new being. However, this process has both advantages and disadvantages, which motivates continuous innovation in the field.