Indole-3-Acetic Acid Production by Pseudomonas Fluorescens LC830621.1 under High Temperature Conditions

Abstract

The aim of the present study was to evaluate in vitro indole-3-acetic acid production by Pseudomonas fluorescens LC830621.1 under high temperature conditions. The rhizospheric bacterial strain identified as Pseudomonas fluorescens LC830621.1, which was isolated from rhizosphere of rice soils contaminated with heavy metals, was taken as reference. Growth kinetics of the bacterium was performed at three temperatures (30, 32 and 45°C). Subsequently, in vitro assay of indole-3-acetic acid production was performed at 32 and 45°C for 26 hours. The results show that the production of indole-3-acetic acid by Pseudomonas fluorescens LC830621.1 at 32°C 1.29 from zero hour to 17.15 mg/L up to 26 hours of incubation. While at 45°C the production of the compound was 0.29 at 0 hours up to 7.5 mg/L up to 26 hours. The results obtained infer the ability of Ps. fluorescens LC830621.1 to produce indole-3-acetic acid up to 45°C and the behaviours and adaptation of growth and auxin expression as a compound involved in plant growth promotion.