Comparison of Immunomodulatory Effects of Phyllanthus niruri L. and Toman Fish Albumin Formulations with Phyllanthus urinaria L. and Toman Fish Albumin through Anti-Hepatotoxic Cytokine Regulation Against Male Rattus norvegicus Experiencing CCl4 Toxicity

Abstract

Impaired liver function can be seen from increased levels (SGOT-SGPT) due to proinflammatory cytokines TNF-α, (iL-1) and (iL-6) which inhibit hormone production (PDGF) in the process of liver tissue regeneration. The formulation of Phyllanthus niruri L. and Phyllanthus urinaria L. combined with toman fish albumin is a combination that can work synergistically in suppressing the production of proinflammatory molecules, because the combination contains compounds that are antioxidants. A total of 24 male Rattus norvegicus were divided into four treatment groups, namely: negative control (healthy), CCl4-induced positive control, dose 1 formulation and dose 2 formulation. Male rattus norvegicus induced CCl4 of 3 ml/kg intraperitoneally, to describe impaired liver function. Male rattus norvegicus was given formula (Phyllanthus niruri L. with Toman fish albumin) and (Phyllanthus urinaria L. and Toman fish albumin) orally at a dose of 1 (620 mg/200 g body weight: 62 mg/200 g body weight), and dose 2 (1240 mg/200 g body weight: 124 mg/200 g body weight) for 14 days. SGOT-SGPT levels were analyzed with a flowcytometer on the first day after acclimatization and day 14, and analysis of proinflammatory cytokines (TNF-α + iL-1 + iL-6) was performed with flow cytometry. This study reported that the combination of Phyllanthus niruri L formula with toman fish albumin (PNA) and Phyllanthus urinaria L formula with toman fish albumin (PUA) was able to show striking hepatoprotective effects through a significant decrease in SGOT-SGPT levels in the blood of male Rattus norvegicus and decreased proinflammatory cytokine profiles in Rattus norvegicus liver cells males exposed to CCl4. In addition, the antioxidant defenses of PNA and PUA formulas almost completely prevent the occurrence of degeneration, necrosis, fibrosis and steatosis in liver cells. So that the combination of PNA and PUA formulas given has the potential to be used as an alternative to hepatitis drugs.